氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响

doi:10.11843/j.issn.0366-6964.2014.11.021

畜牧兽医学报 ›› 2014, Vol. 45 ›› Issue (11): 1888-1894.doi: 10.11843/j.issn.0366-6964.2014.11.021

氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响

徐刚，王金明，马淑慧，詹雅琦，马海利^*

（山西农业大学动物科技学院，太谷 030801）

收稿日期:2014-03-13 出版日期:2014-11-23 发布日期:2014-11-23
通讯作者: 马海利，教授，E-mail：mahaili1718@126.com
作者简介:徐刚（1987-），男，山西大同人，硕士，主要从事动物传染病的诊断与防治研究， E-mail：xugangayu@163.com
基金资助:
山西省高等学校优秀青年学术带头人支持计划（2011）

Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse

XU Gang，WANG Jin-ming，MA Shu-hui，ZHAN Ya-qi，MA Hai-li^*

(College of Animal Science and Veterinary Medicine，Shanxi Agricultural University，Taigu 030801，China)

Received:2014-03-13 Online:2014-11-23 Published:2014-11-23

摘要/Abstract

摘要：

为探讨氟化钠对小鼠脾淋巴细胞细胞外信号调节蛋白激酶(ERK)通路相关基因及蛋白表达的影响，将体内不同氟质量浓度染毒鼠的脾淋巴细胞和体外不同氟浓度作用的脾淋巴细胞经过细菌脂多糖（LPS）刺激培养后，采用 Real-time PCR和Western blotting检测ras、c-raf、MEK1、ERK1在mRNA水平的转录变化以及ERK1在蛋白质水平的表达差异。结果显示：小鼠脾淋巴细胞ERK通路的激活可被低质量浓度的氟抑制，但随氟质量浓度增加，其抑制趋势逐渐减少而转为促进。表明氟化物可通过影响小鼠脾淋巴细胞ERK通路的激活，从而进一步影响免疫系统的功能。

Abstract:

The current study was undertaken to study the effects of fluoride on the expression of ERK signaling pathway associated gene and proteins in the splenic lymphocytes of mouse.The splenic lymphocytes of the different level of fluoride-treated mouse in vivo and the splenic lymphocytes with the different level of sodium fluoride in vitro were cultured in RPMI-1640 medium with bacterial lipopolysaccharide，then the mRNA level of ras/c-raf/MEK1/ERK1 and the protein expression of ERK1 were detected using the Real-time PCR and Western blotting.The results showed that activation of the ERK pathway in mouse splenic lymphocytes can be inhibited by fluoride in low concentrations，but with increasing fluoride concentration，the degree of inhibition was reduced，ERK pathway activation was turn into promoting.These results suggested that fluoride can affect the activation of ERK pathway in mouse splenic lymphocytes，thus further influence the function of the immune system.

中图分类号:

S859.81

徐刚，王金明，马淑慧，詹雅琦，马海利. 氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响[J]. 畜牧兽医学报, 2014, 45(11): 1888-1894.

XU Gang，WANG Jin-ming，MA Shu-hui，ZHAN Ya-qi，MA Hai-li. Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(11): 1888-1894.

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氟对小鼠脾淋巴细胞ERK通路相关基因及蛋白质表达的影响

Effects of Fluoride on the Expression of ERK Signaling Pathway Associated Gene and Proteins in the Splenic Lymphocytes of Mouse

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